Primary cell isolation from tissues was the first step in the in vitro culture process. They enabled the examination of physiological processes outside of the body due to their unique tissue-specific features. Their limited lifespan, however, posed a significant challenge. In order to perform long-term investigations, it required frequent isolation from tissues. This spurred scientists to...

Cancer research is moving at an unparalleled speed, driven by the pressing need to better understand tumor biology and enhance therapy results. The transition from 2D immortalized cell lines to more sophisticated and physiologically realistic models such as cancer spheroids lies at the heart of these advances. Researchers are increasingly using advances in cancer  Cell Culture Media ...

Primary cells have led to significant advancements in research. With its numerous benefits and diverse applications, the primary cell culture has become the focal point of in vitro research. The source of cells in terms of species and tissue is essential. Although human primary cells are preferable in research, their limited availability and requirement for disease-specific models often...

Before choosing cancer cell lines for your experiments, it’s essential to evaluate a few critical factors. Selecting the right cell line impacts the reliability and relevance of your results. Here are five key questions to consider to ensure that your chosen Cancer Cell Line is credible, contamination-free, biologically appropriate, genetically stable, and suitable for your specific...

The liver is essential for protein synthesis, drug metabolism,and bile production. The rising prevalence of liver diseases has spurred substantial research on hepatic cells. In vitro studies particularly utilize primary liver hepatocytes, the epithelial cells of liver parenchyma. They constitute 60% of the liver cell population and 80% of tissue volume. As suitable in vitro models, they have...

Kidneys have an essential role to play in body homeostasis and eliminating body waste. It has been a vital focus for understanding how the drugs interact and affect them. Once they enter the bloodstream, the kidneys are responsible for clearing them out of the system. Even before we move to animal models or preclinical studies, it is necessary to test them on epithelial cells (EC). Primary...

Primary cells are the backbone of biomedical research. Species-specific primary cells have acquired focus from the scientific community owing to the use of animal models. They have accelerated the pace of research and provided valuable information. Their applications in diverse fields range from basic research to drug development. When comparing primary cells vs cell lines in species-specific...

Human primary endothelial cells have been employed extensively in pathophysiology and therapeutic research. These cells are the basic unit of any blood vessel—capillaries, arteries, and veins. As a core component of the circulatory system, they are integrated into each organ. Their role extends from being a mere barrier to regulating tissue homeostasis, vessel integrity, disease...

Mouse Lung epithelial cells are crucial for respiratory function. Located at the interface between the environment and the tissue, they act as protective shields. They also participate in fluid balance, immune response, surfactant synthesis, repair processes, particulate clearance, and disease pathogenesis. Due to the changes in the structure of the lungs during the respiratory cycle, they are...